The GE Pharmacia fast protein liquid chromatography (FPLC) system is used for methods development and the purification of large quantities of various bimolecular (i.e.- protein and DNA). The system includes two pumps capable of continuous flow to the purification columns (vary by user) - a peristaltic pump may be used for column equilibration- a UV monitor to detect bimolecular on the column- a mixer to produce elution gradients- motor valves may be used to assist with sample injection- column selection or flow reversal- and a controller with an integration function for computerized operation of various programs directing flow to the pumps and controlling the motor valves. A chart recorder is the typical mode of run documentation.
...a classic that stands the test of time and will supersede the trendiness of the age...
|Isocratic mode||0.05?20 ml/min in steps of 10 ul/min|
|Gradient mode||0.1?20 ml/min in steps of 10 ?l/min|
|Pressure Range||0?5 MPa (50 bar- 725 psi)|
|Pressure pulsation||Max. 6 percent (dP/P) during pump stroke|
|Less than 10 ml/min||Max. 10cP|
|More Than 10 ml/min||Max. 5cP|
|H x W x H inch||14.6 x 18.9 x 18.5|